Multisizer 4e

Multisizer 4e

The Multiszier 4e COULTER COUNTER detects cell size and volume changes event if they happen over a few seconds or in a period of several hours. Change in cell volume is an important factor involved in many biological processes such as Cell Growth, Cell Cycles, Cell Death, Compensation for Osmotic Stress, Pathogenesis and Phagocytosis.

Cell and Particle Characterization | Benefits for selecting the Multisizer 4e

With the lower size limit of 0.2 microns (200 nm), the Multisizer 4e can be used to count, size and provide mass distribution for organelles such as mitochondria. The Multisizer 4e is an important tool in your cellular research.

Accurate metering device determines cell concentration so you know the exact analyzed volume

Cell, volume, size and concentration (counts) can be measured

Only method that measures a particle "volumetrically" and gives a three-dimensional measure of particle size

Measures one cell/particle at a time

Overall sizing range of 0.2 um to 1600 umVisual particle trending

Increased dynamic range and resolution

Multisizer 4e COULTER COUNTER Specifications

Overall Particle Size Range 0.2 µm to 1600 µm in diameter. 0.033 fL to 2.145 x 109 fL or µm3 in volume
Aperture Diameter 10 µm to 2000 µm apertures (nominal diameters)
Aperture Range Standard 1:30 (by diameter)          Total 1:40 (by diameter)               Standard 1:27,000 (by volume)       Total 1:64,000 (by volume)
Resolution User selectable
Number of Channels Pulse data is digitized and can be processed to achieve up to 400 size channels for a selected pulse range. Number of channels and range can be reprocessed as necessary
Electrolyte Solutions All aqueous and non-aqueous electrolyte solutions recommended for use with aperture technology will be suitable for use with the Multisizer 4e. Electrolytes should be compatible with glass, fluoropolymers, fluoroelastomers and stainless steel
Digital Pulse Processor Proprietary high-speed digitization of the signal
Pulse Data Time stamped pulses up to 525,000 per signal analysis
Size Distribution Data Size distribution by diameter, volume and area for number, number%, number/ml, volume, volume%, volume/ml, surface area, surface area% and surface area/ml
Pulse Distribution Data Pulse distribution by time, sequence and width for pulse height diameter, pulse height volume, pulse height volt, pulse width, pulse area, average pulse height diameter, average pulse height volume and average pulse width. Number distribution by width.
Linearity ± 1% for diameter        ± 3% for volume
Aperture Current Range 30 µA - 6000 µA in 0.2 µA steps
Aperture Current Accuracy ± 0.4% of setting
Polarity Error Less than 0.5%
Time Mode 0.1 to 999 seconds, selectable in 10 ms increments.Typically, time analysis is 10 to 90 seconds
Total Count Mode 50 to 500,000 counts
Modal Count Mode 10 to 100,000 counts
Volumetric Mode Continuously selectable from 50 µl to 2000 µl
Metering System Mercury-free, wide range metering pump
Volumetric Pump Accuracy Better than 99.5%
Regulatory Compliance The software enables 21 CFR Part 11 compliance
Dimensions Weight and Power (excluding computer) Unpacked weight: 45 kg (99 lb)        Width: 64 cm (25 in)                Depth: 61 cm (24 in)     Height: 51 cm (20 in) Input voltage within set ranges: 100 - 120 VAC; 230 - 240 VAC ± 10%; single phase
Supply Frequency 47 to 63 Hz inclusive
Power Less than 55 volt-amps (watts)
Fuse Types 250 V, IEC (5x20 mm),Time delay (TD), 2.0 A
Environmental Conditions a) This instrument is safe for indoor use only.      b) Installation category: 11           c) Pollution degree: 1
Operating Temperature 5°C to 40°C
Relative Humidity 30% to 85% non-condensing
Altitude Up to 2000 m (6560 ft)


Ratthakarn Nilagupta : 2019-04-20 23:27:29 : Particle Size Analyzer

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Items included with the Multisizer 4e

Part Number Description
B23005 Multisizer 4e Instrument with 100 um Aperture Tube
8546719 Isoton II Diluent, 20L
8546930 Coulter Clenz, 5L
6602798 L20 Standard, nominal 20 um, latex particle (NIST Traceable), 1 x 15 mL
6602796 L10 Standard, nominal 10 um, latex particle (NIST Traceable), 1 x 15mL
A35473 Accuvette, 200 vials & caps (25mL)

 

http://www.beckman.com/particle/instruments/cell-sizing-and-processing/multisizer-4e

 

Applications & Industries:

 

Pharmaceutical QC

Filtration efficiency

Sub-visible particle monitoring and characterization

Bioprocessing monitoring

Food & Beverage QC

Yeast monitoring

Flavor emulsions

Monitoring particle levels

Industrial QC

Toner

Abrasives

Cosmetics

Emulsions

Beads

Powders

Marine Biology

Cell Biology

Microbiology

http://www.beckman.com/particle/instruments/cell-sizing-and-processing/multisizer-4e